Hi,
I did this a few nights ago, 3x4 minute subs unguided with an ED80 and AP.67 focal reducer and CLS filter, down to mag 12.8 I think.
(http://farm3.static.flickr.com/2142/2493378150_29d2e40b45_b.jpg)
Nice one! Very clear.
Nice image Chris however, I think FOV is smaller than you think - I think you have made an error with your labels. I think that what you have labelled as NGC4479 & 4477 are actually 4461 & 4458 & that M88 is actually NGC4473. See image below from SkyMap. The distance between m86 & m88 is greater than 2 degrees whereas m86 to ngc4473 spans about 1 degree.
Cheers, John
(http://homepage.ntlworld.com/john.punnett/graphics/oas/m86area1.jpg)
Nice one Chris. Can we see a non-negative version without the labels as well please.